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人小細(xì)胞肺癌細(xì)胞;NCI-H446

更新時(shí)間:2016-06-21

簡(jiǎn)要描述:

人小細(xì)胞肺癌細(xì)胞;NCI-H446,開始培養(yǎng)保持細(xì)胞濃度在2×10 exp5個(gè)細(xì)胞/ ml和1×10 exp5和1×10 exp6個(gè)細(xì)胞/ ml之間。只用于實(shí)驗(yàn)室研究。不能被用于在臨床檢測(cè)和有關(guān)于人身安全的實(shí)驗(yàn)。

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人小細(xì)胞肺癌細(xì)胞;NCI-H446
形態(tài)特性 上皮樣
生長(zhǎng)特性 貼壁/懸浮生長(zhǎng),混合 
特征特性人小細(xì)胞肺癌細(xì)胞;NCI-H446該細(xì)胞是1982年由Carney D和Gazdar AF等從一位小細(xì)胞肺癌患者的胸腔積液中建立的。細(xì)胞的原始形態(tài)并不具有小細(xì)胞肺癌特征。這個(gè)細(xì)胞株是小細(xì)胞肺癌的生化和形態(tài)學(xué)上的變種,表達(dá)神經(jīng)元*的烯醇酶和腦型肌酸激酶同工酶;左旋多巴脫羧酶、蠶素、抗利尿激素、催產(chǎn)素或胃泌激素釋放肽未達(dá)到可檢測(cè)水平。與正常細(xì)胞相比,該細(xì)胞c-myc DNA序列擴(kuò)增約20倍,RNA增加15倍。zui初傳代培養(yǎng)基用含有5%FBS的RPMI1640,另外添加0.005 mg/ml胰島素、0.01 mg/ml轉(zhuǎn)鐵
培養(yǎng)條件 RPMI 1640 (w/o Hepes) 10%FBS 
傳代方法 1:3~1:9傳代;每周換液2~3次。
傳代情況 C5
凍存條件 基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS 
公司細(xì)胞廣泛用于國(guó)內(nèi)院校的細(xì)胞生物學(xué)研究,他們的很多論文都或多或少使用我們公司的細(xì)胞,作為實(shí)驗(yàn)項(xiàng)目研究
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