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小鼠睪丸畸胎瘤細胞;P19

更新時間:2016-06-17

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小鼠睪丸畸胎瘤細胞;P19,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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小鼠睪丸畸胎瘤細胞;P19
形態特性 上皮樣
生長特性 貼壁生長 
特征特性 小鼠睪丸畸胎瘤細胞;P19P19細胞株是從C3H/He小鼠中誘導的惡性畸胎瘤中建立的。該細胞在含有0.1mM 2-巰基乙醇的培養基中可高效率地克隆。該細胞具有多能性,在500nM 維A酸誘導下可以分化成神經和神經膠質樣細胞;在0.5%~1.0%二甲亞砜(DMSO) 存在下,分化形成心臟和骨骼肌樣細胞,但不形成神經或神經膠質樣細胞;在DMSO和維A酸同時存在時,細胞的分化與只有維A酸一樣。
培養條件 α-MEM:Alpha Minimum Essential Medium (with Nucleosides) 10%FBS 
傳代方法 勿使細胞長至匯合狀態,1:10傳代;2~3天1次。
傳代情況 C5
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究。
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