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人宮頸癌細胞;Hela

更新時間:2016-06-15

簡要描述:

人宮頸癌細胞;Hela,開始培養保持細胞濃度在2×10 exp5個細胞/ ml和1×10 exp5和1×10 exp6個細胞/ ml之間。只用于實驗室研究。不能被用于在臨床檢測和有關于人身安全的實驗。

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人宮頸癌細胞;Hela
形態特性 上皮樣
生長特性 貼壁生長 
特征特性 人宮頸癌細胞;HelaHeLa是*個來自人體組織經連續培養獲得的非整倍體上皮樣細胞系,它由Gey GO等在1951年從31歲女性黑人的宮頸癌組織建立。經原始組織切片重新觀察,Jones等將其診斷為腺癌。已知該細胞系含有人乳頭狀瘤病毒HPV18序列,需在2級生物安全防護臺操作。該細胞角蛋白陽性,p53表達量較低,但表達正常水平的pRB(視網膜母細胞瘤抑制因子)。
培養條件 RPMI 1640 (w/o Hepes) 10%FBS 
傳代方法 1:2~1:6傳代;2~3天1次。
傳代情況 P130
凍存條件 基礎培養基+5%DMSO+20%FBS 
公司細胞廣泛用于國內院校的細胞生物學研究,他們的很多論文都或多或少使用我們公司的細胞,作為實驗項目研究。
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